Detailed explanation of storage methods for adenosine deaminase (ADA)

Adenosine deaminase (ADA), as a key enzyme in purine metabolism, plays an important role in the development of the immune system, diagnosis of liver diseases, and differentiation of tuberculous pleurisy. The activity detection relies on the stability of the reagents, and the scientific storage method directly affects the accuracy of the detection results. This article will systematically analyze the storage points of adenosine deaminase from four dimensions: short-term frequent use, long-term storage, packaging selection, and storage environment management, based on product characteristics and operating standards.

1、 Storage standards for short-term frequent use scenarios

For laboratories that require frequent testing, it is recommended to store adenosine deaminase reagents in a refrigerated environment between 2 ℃ and 4 ℃. This temperature range can effectively balance enzyme activity and stability. Taking the ELISA kit as an example, after opening, the enzyme-linked immunosorbent assay (ELISA) coated plate should be immediately placed in a sealed bag and stored in a refrigerator at 4 ℃ to avoid repeated freeze-thaw cycles leading to enzyme activity decline. Special attention should be paid to the possibility of salt precipitation in concentrated detergent at low temperatures. Therefore, it is important to ensure sufficient dissolution before use and to use sterile water during the dilution process to prevent external ammonia interference with the test results.

2、 Long term storage conditions and operating points

When adenosine deaminase needs to be stored for a long time, its shelf life can be extended at a low temperature of -20 ℃. Special attention should be paid to using enzyme preparations as soon as possible after each thawing to avoid secondary freezing and thawing. For example, experimental data showed that the ADA enzyme activity loss rate after three freeze-thaw cycles was as high as 40%.

3、 Packaging protection and packaging specifications

The selection of packaging materials directly affects the stability of adenosine deaminase. It is recommended to use light proof and moisture-proof aluminum foil bags or sealed tubes for packaging. For large volume adenosine deaminase preparations, they should be divided according to the usage amount, and only one tube should be thawed for each use. The remaining part should continue to be frozen and stored. In addition, it is necessary to avoid mixing with volatile chemical reagents.

4、 Storage environment control key

The storage environment must strictly control temperature, humidity, and lighting conditions. Refrigeration equipment should be equipped with a temperature monitoring system to ensure that the temperature fluctuation range does not exceed ± 2 ℃; The refrigeration equipment needs to be defrosted regularly to avoid the formation of ice crystals that may affect the stability of the reagents. The reagent kit containing adenosine deaminase should be stored in a dry and dark place to avoid coexistence with volatile chemical reagents. For example, the enzyme label plate in the ELISA kit needs to be used after being balanced at room temperature for 15-30 minutes. If the humidity in the storage environment is too high, the Flat noodles may be deformed due to moisture absorption. In addition, the reagent should be used as soon as possible after opening, and the concentrated standard should be discarded immediately after dilution, and cannot be repeatedly frozen.

The storage management of ADA reagents should take into account temperature control, light protection, and storage environment management. Through a scientific storage strategy, not only can the long-term stability of the reagents be ensured, but reliable enzyme activity data support can also be provided for clinical diagnosis. Hubei Xindesheng Material Technology Co., Ltd. has established a professional enzyme preparation research and development team, and has successfully developed glutamate dehydrogenase, uricase, adenosine deaminase, cholesterol oxidase, etc. If you need this type of product, please click on the official website to learn more details or contact me!